Local Cold Stress to the Soles Induces Retroplacental Hemorrhage in Rats

 

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ABSTRACT

The aim of this study was to investigate the effect of local cold stress to the soles on placental histology in rats. Pregnant rats were subjected to a cold provocation (0°C, 12°C) and room temperature (23°C) for 15 and 30 minutes. During cold stimulation and at the control temperature, uterine blood flow and uterine contraction were measured simultaneously. Histological studies of the placentas of these rats were also performed. Cold stress at 0°C and 12°C significantly decreased uterine blood flow (P < .005, P < .02) compared with controls (23°C). The decrease in uterine blood flow was greater at 0°C than at 12°C stress. Cold-induced stress (0°C) also evoked an isometric tension with increased frequency and amplitude in the rat uterus (P < .003, P < .0002) compared with controls (23°C). Stimulation at 12°C did not induce any uterine contractions. Placental histology of rats stressed at 0°C revealed hemorrhages into the decidua basalis. These findings suggest that local cold stress decreases uterine blood flow and increases uterine contraction, resulting in retroplacental hemorrhage in rats. This model may account for human abruptio placentae.

REFERENCES

• 1 Spinillo A, Capuzzo E, Colonna L. Factors associated with abruptio placentae in preterm deliveries.  Acta Obstet Gynaecol Scand . 1994;  73 307-312
  PubMedGoogle Scholar
• 2 Sher G. Pathogenesis and management of uterine inertia complicating abruptio placentae with consumption coagulopathy.  Am J Obstet Gynecol . 1977;  129 164-170
  PubMedGoogle Scholar
• 3 Saftlas A E, Olson D R, Atrash H K. National trends in the incidence of abruptio placentae, 1979-1987.  Obstet Gynecol . 1991;  78 1081-1086
  PubMedGoogle Scholar
• 4 DeCherney A H, Pernoll M L. Current Obstetrics and Gynecologic Diagnosis and Treatment. Premature Separation of the Placenta (Abruptio Placentae, Ablatio Placentae, Accidental Hemorrhage) 8th ed. Norwalk, CT: Appleton & Lange 1992: 400-404
  Google Scholar
• 5 Converse Jr L R, Jacobsen T N, Toto R D. Sympathetic overactivity in patients with chronic renal failure.  N Engl J Med . 1992;  327 1912-1918
  PubMedGoogle Scholar
• 6 Daly P A, Landsberg L. Hypertension in obesity and NIDDM. Role of insulin and sympathetic nervous system.  Diabetes Care . 1991;  14 240-248
  PubMedGoogle Scholar
• 7 Schobel H P, Fischer T, Heuszer K. Preeclampsia-a state of sympathetic overactivity.  N Engl J Med . 1996;  335 1480-1485
  CrossrefPubMedGoogle Scholar
• 8 Scrogin K E, Hatton D C, Chi Y. Chronic nitric oxide inhibition with L-NAME: effects on autonomic control of the cardiovascular system.  Am J Physiol . 1998;  274 R367-R374
  PubMedGoogle Scholar
• 9 Hines E A, Brown G E. The cold pressor test for measuring the reactability of the blood pressure: data concerning 571 normal and hypertensive subjects.  Am Heart J . 1936;  11 1-9
  CrossrefPubMedGoogle Scholar
• 10 Milne B. Lower body nerve stretch: a role in essential hypertension or pre-eclampsia?.  Med Hypotheses . 1996;  47 333-335
  PubMedGoogle Scholar
• 11 Cavanagh D, Rao P S, Tsai C C. Experimental toxemia in the pregnant primate.  Am J Obstet Gynecol . 1977;  128 75-85
  PubMedGoogle Scholar
• 12 Coombs C A, Kart M A, Kitzmiller J L. Experimental preeclampsia produced by constriction of the lower aorta: validation with longitudinal blood pressure measurements in conscious rhesus monkeys.  Am J Obstet Gynecol . 1993;  169 215-223
  CrossrefPubMedGoogle Scholar
• 13 Meekins J W, Pijnenborg R, Hanssens M. A study of placental bed spiral arteries and trophoblast invasion in normal and severe preeclampsia.  Br J Obstet Gynaecol . 1994;  101 669-674
  CrossrefPubMedGoogle Scholar
• 14 Zhou Y, Chiu K, Brescia R J. Increased depth of trophoblast invasion after chronic constriction of lower aorta in rhesus monkey.  Am J Obstet Gynecol . 1993;  169 224-229
  PubMedGoogle Scholar
• 15 Kanayama N, Tsujimura R, She L. Cold-induced stress stimulates the sympathetic nervous system, causing hypertension and proteinuria in rats.  J Hypertens . 1997;  15 383-389
  CrossrefPubMedGoogle Scholar
• 16 Belayet H M, Kanayama N, Khatun S. Decreased renal and hepatic blood flow with pre-eclampsia-like histologic changes was obtained by stimulation of the celiac ganglion with LPS.  Am J Perinatol . 1998;  15 109-114
  PubMedGoogle Scholar
• 17 Machino H, Kobayashi H, Hayashi K. Nitric oxide is involved in the inhibitory action of cholecystokinin octapeptide (CCK-OP) on proximal colonic motility.  Regul Pept . 1997;  69 47-52
  CrossrefPubMedGoogle Scholar
• 18 Naeye R L, Harkness W L, Utts J. Abruptio placentae and perinatal death: a prospective study.  Am J Obstet Gynecol . 1997;  128 740-746
  PubMedGoogle Scholar
• 19 Valera E D. Abruptio placentae.  Am J Obstet Gynecol . 1968;  100 599-606
  PubMedGoogle Scholar
• 20 Zhang L, Pearce W, Longo L D. Noradrenaline-mediated contractions of ovine uterine artery: role of inositol 1,4, 5-trisphosphate.  Eur J Pharmacol . 1995;  289 375-382
  PubMedGoogle Scholar
• 21 Rocha L RM, Calixto S L, Ladosky W. Hormonal influence on the response of the uterus of the Brazilian marsupial Didelphis albiventris to adrenergic drugs.  Gen Comp Endocrinol . 1985;  58 179-185
  CrossrefPubMedGoogle Scholar
• 22 Borell U, Fernstrom I, Ohlson L. Influence of uterine contractions on the uteroplacental blood flow at term.  Am J Obstet Gynecol . 1965;  93 45-57
  PubMedGoogle Scholar
• 23 Eskes T A B K. Abruptio placentae. A ``classic'' dedicated to Elizabeth Ramsey.  Eur J Obstet Gynecol Reprod Biol . 1997;  75 63-70
  CrossrefPubMedGoogle Scholar
• 24 Sherer D M, D'Amico M L, Warshal D P. Recurrent mild abruptio placentae occurring immediately after repeated electroconvulsive therapy in pregnancy.  Am J Obstet Gynecol . 1991;  165 652-653
  PubMedGoogle Scholar