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Horm Metab Res 2010; 42(1): 14-22
DOI: 10.1055/s-0029-1233480
Humans, Clinical

© Georg Thieme Verlag KG Stuttgart · New York

Lack of Significant Effects of the Type 2 Diabetes Susceptibility Loci JAZF1, CDC123/CAMK1D, NOTCH2, ADAMTS9, THADA, and TSPAN8/LGR5 on Diabetes and Quantitative Metabolic Traits

D. Schleinitz 1 [*] , A. Tönjes 2 , 3 [*] , Y. Böttcher 2 , K. Dietrich 1 , B. Enigk 1 , M. Koriath 2 , G. H. Scholz 4 , M. Blüher 2 , E. Zeggini 5 , M. I. McCarthy 5 , 6 , 7 , P. Kovacs 1 , M. Stumvoll 2
  • 1Interdisciplinary Centre for Clinical Research, University of Leipzig, Leipzig, Germany
  • 2Department of Medicine, University of Leipzig, Leipzig, Germany
  • 3Coordination Centre for Clinical Trials, University of Leipzig, Leipzig, Germany
  • 4St. Elisabeth Hospital, Medical Department, Leipzig, Germany
  • 5Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK
  • 6Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, UK
  • 7Oxford NIHR Biomedical Research Centre, Oxford, UK
Further Information

Publication History

received 03.03.2009

accepted 15.06.2009

Publication Date:
10 August 2009 (online)

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Abstract

Recently, several novel loci reaching genome-wide significance levels for type 2 diabetes (T2D) were identified through a meta-analysis of three genome-wide scans and large-scale follow-up. The aim of our study was to investigate the association of these loci with T2D and related subphenotypes in two cohorts from Germany. We performed an association study of 9 SNPs in or around JAZF1, CDC123/CAMK1D, NOTCH2, BCL11A, ADAMTS9, VEGFA, DCD, THADA, and TSPAN8/LGR5 with T2D and related quantitative traits (fasting insulin and glucose, indices derived from OGTT) in the isolated population of Sorbs (205 cases and 695 controls) and in a mixed German population (Leipzig) (938 subjects with and 918 without T2D). None of the variants was associated with T2D, but the meta-analysis of both cohorts revealed a modest trend of association of rs7578597 in THADA with T2D (p=0.055). Furthermore, Sorbian subjects homozygous for the rs7578597 T-allele had lower mean 30-minute plasma insulin when compared with carriers of the C-allele (p<0.05). The T-allele was also nominally associated with higher fasting plasma glucose in the Leipzig cohort (p<0.05). Although several other SNPs showed some evidence for association with T2D-related traits the effects were not replicated within our study. Associations of the T2D-risk alleles with T2D or related subphenotypes were overall very weak in the ∼2 700 subjects studied. This is compatible with the modest effect size of these “second sweep” variants, which will require large-scale association studies on quantitative traits to clarify their role in the pathophysiology of T2D.

Key words

type 2 diabetes - genetics - single nucleotide polymorphism

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1 These authors contributed equally to the manuscript.

Correspondence

M. Stumvoll

Department of Medicine

University of Leipzig

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Email: Michael.Stumvoll@medizin.uni-leipzig.de

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