Hostname: page-component-848d4c4894-pftt2 Total loading time: 0 Render date: 2024-05-22T11:26:04.726Z Has data issue: false hasContentIssue false
  • English
  • Français

Origins of the electroretinogram oscillatory potentials in the rabbit retina

Published online by Cambridge University Press:  01 July 2004

CUN-JIAN DONG ,
PETER AGEY  and
WILLIAM A. HARE
CUN-JIAN DONG
Affiliation:
Department of Biological Sciences, Allergan Pharmaceuticals, Irvine
PETER AGEY
Affiliation:
Department of Biological Sciences, Allergan Pharmaceuticals, Irvine
WILLIAM A. HARE
Affiliation:
Department of Biological Sciences, Allergan Pharmaceuticals, Irvine
Get access Rights & Permissions [Opens in a new window]

Abstract

The electroretinogram (ERG) oscillatory potential (OP) is a high-frequency, low-amplitude potential that is superimposed on the rising phase of the b-wave. It provides noninvasive evaluation of inner retina function in vivo and is a useful tool in basic research as well as in the clinic. While the OP is widely believed to be generated mainly by activity of the inner retina, the exact underlying neural mechanisms are not well understood. We have investigated the retinal mechanisms that underlie OP generation in Dutch-belted rabbits. The OP was isolated by band-filtering (100–1000 Hz) ERG signals. We used pharmacological agents that block specific transmitter receptors or voltage-gated channels in order to examine contributions of various retinal mechanisms to OP generation. Our results show that the OP elicited by a bright brief flash can be classified into early, intermediate, and late subgroups that are likely generated mainly by photoreceptors, action-potential-independent, and action-potential-dependent mechanisms in the ON pathway of the inner retina, respectively. ON bipolar cells themselves make only a small direct contribution to OP generation, as do horizontal cells and neurons in the OFF pathway.

Keywords

Oscillatory potentialERGRabbit
Type
Research Article
Information
Visual Neuroscience , Volume 21 , Issue 4 , July 2004 , pp. 533 - 543
Copyright
2004 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Blanco, R. & de la Villa, P. (1999). Ionotropioc glutamate receptors in isolated horizontal cells of the rabbit retina. European Journal of Neuroscience 11, 863–873.Google Scholar
Bresnik, G.H., Korth, K., Groo, A., & Palta, M. (1984). Electroretinographic oscillatory potentials predict progression of diabetic retinopathy. Preliminary report. Archives of Ophthalmology 102, 1307–1311.CrossRefGoogle Scholar
Bui, B.V., Armitage, J.A., & Vingrys, A.J. (2002). Extraction and modeling of oscillatory potentials. Documenta Ophthalmologica 104, 17–36.CrossRefGoogle Scholar
Chen, S. & Diamond, J.S. (2002). Synaptically released glutamate activates extrasynaptic NMDA receptors on cells in the ganglion cell layer of rat retina. Journal of Neuroscience 22, 2165–2173.Google Scholar
Derr, P.H., Meyer, A.U., Haupt, E.J., & Brigell, M.G. (2002). Extraction and modeling of the oscillatory potential: Signal conditioning to obtain minimally corrupted oscillatory potentials. Documenta Ophthalmologica 104, 37–55.CrossRefGoogle Scholar
DeVries, S.H. (2000). Bipolar cells use kainite and AMPA receptors to filter visual information into separate channels. Neuron 28, 847–856.CrossRefGoogle Scholar
Dowling, J.E. (1987). The Retina: An Approachable Part of the Brain. Cambridge, Massachusetts: Harvard University Press.
Grant, G.B. & Werblin, F.S. (1994). Low-cost data acquisition and analysis programs for electrophysiology. Journal of Neuroscience Methods 55, 89–98.CrossRefGoogle Scholar
Guite, P. & Lachapelle, P. (1990). The effect of 2-amino-4-phosphonobutyric acid on the oscillatory potentials of the electroretinogram. Documenta Ophthalmologica 75, 125–133.CrossRefGoogle Scholar
Hanitzsch, R., Karbaum, R., & Lichtenberger, T. (1998). Do horizontal cells contribute to the rabbit ERG? Documenta Ophthalmologica 97, 57–66.Google Scholar
Hare, W.A. & Ton, H. (2002). Effects of APB, PDA, and TTX on ERG responses recorded using both multifocal and conventional methods in monkey. Documenta Ophthalmologica 105, 189–222.CrossRefGoogle Scholar
Jensen, R.J. (1991). Involvement of glycinergic neurons in the diminished surround activity of ganglion cells in the dark-adapted rabbit retina. Visual Neuroscience 6, 43–53.CrossRefGoogle Scholar
Karwoski, C. & Kawasaki, K. (1991). The oscillatory potentials. In Principles and Practice of Clinical Electrophysiology of Vision, ed. Heckenlively, J.R. & Arden, G.B., pp. 125–128. St. Louis, Missouri: Mosby-Year Book.
Lachapelle, P., Little, J., & Palomeno, R. (1983). The electroretinogram in congenital stationary night blindness with myopia. Investigative Ophthalmology and Visual Science 24, 442–450.Google Scholar
Lachapelle, P., Little, J., & Roy, M.S. (1990). The electroretinogram in Stargart's disease and fundus flavimaculatus. Documenta Ophthalmologica 73, 395–404.Google Scholar
McCall, M.A., Lukasiewicz, P.D., Gregg, R.G., & Peachey, N.S. (2002). Elimination of the rho1 subunit abolishes GABA(C) receptor expression and alters visual processing in the mouse retina. Journal of Neuroscience 22, 4163–4174.Google Scholar
Rangaswamy, N.V., Hood, D.C., & Frishman, L.J. (2003). Regional variations in local contributions to the promate photopic flash ERG: Revealed using the slow-sequence mfERG. Investigative Ophthalmology and Visual Science 44, 3233–3247.CrossRefGoogle Scholar
Shiells, R.A. & Falk, G. (1999). Contribution of rod, on-bipolar, and horizontal cell light responses to the ERG of dogfish retina. Visual Neuroscience 16, 503–511.Google Scholar
Slaughter, M.M. & Miller, R.F. (1981). 2-amino-4-phosphonobutyric acid: A new pharmacological tool for retina research. Science 211, 182–185.CrossRefGoogle Scholar
Wachtmeister, L. (1980). Further studies of the chemical sensitivity of the oscillatory potentials of the electroretinogram (ERG). I. GABA- and glycine antagonists. Acta Ophthalmologica (Copenhagen) 58, 712–725.Google Scholar
Wachtmeister, L. (1998). Oscillatory potentials in the retina: What do they reveal. Progress in Retinal and Eye Research 17, 485–521.CrossRefGoogle Scholar
Wachtmeister, L. & Dowling, J. (1978). The oscillatory potentials of the mudpuppy retina. Investigative Ophthalmology and Visual Science 17, 1176–1188.Google Scholar
Yonemura, D., Aoki, T., & Tsuzuki, K. (1962). Electroretinogram in diabetic retinopathy. Archives of Ophthalmology 68, 19–24.CrossRefGoogle Scholar
Yuste, R., Majewska, A., Cash, S.S., & Denk, W. (1999). Mechanisms of calcium influx into hippocampal spines: Heterogeneity among spines, coincidence detection by NMDA receptors, and optical quantal analysis. Journal of Neuroscience 19, 1976–1987.Google Scholar