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Effects of CD4+CD25+Foxp3+regulatory T cells on early Plasmodium yoelii 17XL infection in BALB/c mice

Published online by Cambridge University Press:  02 July 2009

GUANG CHEN ,
JUN LIU ,
QING-HUI WANG ,
YI WU ,
HUI FENG ,
WEI ZHENG ,
SHENG-YU GUO ,
DONG-MEI LI ,
JI-CHUN WANG  and
YA-MING CAO
GUANG CHEN
Affiliation:
Department of Immunology, College of Basic Medical Sciences, China Medical University, No.92 Beier Road, Heping District, Shenyang, China Department of Parasitology, College of Basic Medical Sciences, Jamusi University, Jamusi 154000, China
JUN LIU
Affiliation:
Department of Immunology, College of Basic Medical Sciences, China Medical University, No.92 Beier Road, Heping District, Shenyang, China
QING-HUI WANG
Affiliation:
Department of Immunology, College of Basic Medical Sciences, China Medical University, No.92 Beier Road, Heping District, Shenyang, China
YI WU
Affiliation:
Department of Internal Medicine, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China
HUI FENG
Affiliation:
Department of Immunology, College of Basic Medical Sciences, China Medical University, No.92 Beier Road, Heping District, Shenyang, China
WEI ZHENG
Affiliation:
Department of Immunology, College of Basic Medical Sciences, China Medical University, No.92 Beier Road, Heping District, Shenyang, China
SHENG-YU GUO
Affiliation:
Department of Rheumatology and Immunology, Second Clinical College, China Medical University, No.36 Sanhao Street, Heping District, Shenyang, China, 110004
DONG-MEI LI
Affiliation:
Shanghai Institute of Biological Products, 1262YanAn Road (W), Shanghai 200000, China
JI-CHUN WANG
Affiliation:
Department of Pathogenic Biology, College of Basic Medical Sciences, China Medical University, No. 92 Beier Road, Heping District, Shenyang, China
YA-MING CAO*
Affiliation:
Department of Immunology, College of Basic Medical Sciences, China Medical University, No.92 Beier Road, Heping District, Shenyang, China
*
*Corresponding author: Department of Immunology, College of Basic Medical Sciences, China Medical University, No. 92 Beier Road, Heping District, Shenyang, China. Tel: +86 24 23256666 5346. Fax: +86 24 23264417. E-mail address: ymcao@mail.cmu.edu.cn
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Summary

The outcome of Plasmodium yoelii 17XL-infected BALB/c and DBA/2 mice, ranging from death to spontaneous cure, respectively, depends largely on the establishment of effective pro-inflammatory type 1 responses during the early stages of infection and associates with CD4+CD25+Foxp3+regulatory T cells (Tregs). Here, effects of Tregs were analysed on early P. yoelii 17XL infection in BALB/c and DBA/2 mice. In vivo depletion of Tregs significantly reversed the inhibited establishment of effective pro-inflammatory type 1 responses in BALB/c mice, indicating that this cell population contributed to the suppression of T-cell function in malaria. Moreover, the proportion and absolute numbers of IL-10-secreting Tregs in BALB/c mice were significantly higher than that found in DBA/2 mice by intracytoplasmic staining, and IL-10 production was correlated with the Tregs population. In addition, in vivo Tregs depletion decreased the production of IL-10 and the apoptosis of CD4+ T cells. Consistently, IL-10R blockade also had the same effect as that of Tregs depletion in P. yoelii 17XL-infected BALB/c mice. Our data demonstrate that Tregs perhaps have an important role in regulating pro-inflammatory type 1 responses in an IL-10-dependent manner and induce CD4+ T cell apoptosis during the early stage of P. yoelii 17XL infection.

Keywords

CD4+CD25+Foxp3+regulatory T cellsPlasmodium yoelii 17XLearly infection
Type
Research Article
Information
Parasitology , Volume 136 , Issue 10 , September 2009 , pp. 1107 - 1120
Copyright
Copyright © Cambridge University Press 2009

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