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Developmental competence and expression of the MATER and ZAR1 genes in immature bovine oocytes selected by brilliant cresyl blue

Published online by Cambridge University Press:  26 November 2009

Gustavo Bruno Mota ,
Ribrio Ivan Tavares Pereira Batista ,
Raquel Varella Serapião ,
Mariana Cortes Boité ,
João Henrique Moreira Viana ,
Ciro Alexandre Alves Torres  and
Luiz Sergio de Almeida Camargo
Gustavo Bruno Mota
Affiliation:
Departamento de Zootecnia, Universidade Federal de Viçosa, Av. P H Rolfs s/n, Viçosa, MG, 36571–000, Brazil.
Ribrio Ivan Tavares Pereira Batista
Affiliation:
Embrapa Gado de Leite, Juiz de Fora, MG, 36038–330, Brazil.
Raquel Varella Serapião
Affiliation:
Embrapa Gado de Leite, Juiz de Fora, MG, 36038–330, Brazil.
Mariana Cortes Boité
Affiliation:
Embrapa Gado de Leite, Juiz de Fora, MG, 36038–330, Brazil.
João Henrique Moreira Viana
Affiliation:
Embrapa Gado de Leite, Juiz de Fora, MG, 36038–330, Brazil.
Ciro Alexandre Alves Torres
Affiliation:
Departamento de Zootecnia, Universidade Federal de Viçosa, Av. P H Rolfs s/n, Viçosa, MG, 36571–000, Brazil.
Luiz Sergio de Almeida Camargo*
Affiliation:
Embrapa Gado de Leite, Juiz de Fora, MG, 36038–330, Brazil.
*
All correspondence to: Luiz S. A. Camargo. Embrapa Gado de Leite, Juiz de Fora, MG, 36038–330, Brazil. Tel: +55 32 32494800. Fax: +55 32 32494701. e-mail: camargo@cnpgl.embrapa.br
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Summary

The objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 °C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BCB+ (coloured cytoplasm) or BCB− (colourless cytoplasm). The control group was submitted to in vitro maturation (IVM) immediately after morphological selection and holding control group COCs were exposed to mDPBS without BCB but in the same incubation conditions of BCB+ and BCB− group. The COCs of all groups were submitted to IVM, in vitro fertilization (IVF) and in vitro culture (IVC). Cleavage rate (72 h post-insemination) was similar between control (65.3%) and BCB+ (64.4%) groups, but greater than (p < 0.05) holding control (49.8%) and BCB− (51.3%) groups. Blastocyst rate (192 h post-insemination) was not different between BCB+ (18.5%) and control (16.3%) groups, but greater (p < 0.05) than BCB− (8.4%) group. No difference was found for blastocyst rate between holding control group (14.2%), control and BCB+ groups. The relative expression of MATER and ZAR1 genes was evaluated by real-time PCR in immature oocytes collected from the control, holding control, BCB+ and BCB− groups. Despite the relative expression of MATER in holding control, BCB+ and BCB− were down regulated in comparison to control group there was no statistical difference (p > 0.05) in the relative expression of MATER and ZAR1 transcripts among groups. The results indicate that the BCB dye detects immature oocyte populations with different developmental competence, although no improvement in in vitro embryo production using oocytes exposed or not to BCB was observed. Development competence of immature oocytes exposed to BCB does not seem to be associated with variations in the expression of MATER and ZAR1 transcripts.

Keywords

Brilliant cresyl blueBovineGene expressionIn vitro fertilizationOocytes
Type
Research Article
Information
Zygote , Volume 18 , Issue 3 , August 2010 , pp. 209 - 216
Copyright
Copyright © Cambridge University Press 2009

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