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Cyclin D1 protein expression is related to clinical progression in laryngeal squamous cell carcinomas

Published online by Cambridge University Press:  29 June 2007

Pasquale Capaccio ,
Giancarlo Pruneri ,
Nadia Carboni ,
Angelo Virgilio Pagliari ,
Roberto Buffa ,
Antonino Neri ,
Antonio Ottaviani  and
Lorenzo Pignataro
Pasquale Capaccio
Affiliation:
Clinica Otorinolaringoiatrica I, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
Giancarlo Pruneri
Affiliation:
II Servizio di Anatomia Patologica, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
Nadia Carboni
Affiliation:
II Servizio di Anatomia Patologica, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
Angelo Virgilio Pagliari
Affiliation:
Clinica Otorinolaringoiatrica I, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
Roberto Buffa
Affiliation:
II Servizio di Anatomia Patologica, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
Antonino Neri
Affiliation:
Servizio di Ematologia, Laboratorio di Ematologia Sperimentale e Genetica Molecolare, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
Antonio Ottaviani
Affiliation:
Clinica Otorinolaringoiatrica I, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
Lorenzo Pignataro*
Affiliation:
Clinica Otorinolaringoiatrica I, Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore, I.R.C.C.S., 20122, Milan, Italy.
*
Address for correspondence: Lorenzo Pignataro, M.D., Clinica Otorinolaringoiatrica I, Ospedale Maggiore, I.R.C.C.S., Via F. Sforza 35, 20122, Milano, Italy. Fax: +39(2)55180809.
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Abstract

The expression of cyclin D1 gene was investigated in 74 laryngeal squamous cell carcinomas (LSCCs) in order to determine its clinical and prognostic value. Overexpression of cyclin Dl was detected immunohistochemically using DCS6 monoclonal antibody on formalin-fixed, paraffin-embedded tissue sections. Cyclin D1 expression was detected in 22 of the 74 cases investigated (30 per cent), thirteen of which presented nodal metastases (59 per cent); of the patients without any detectable cyclin D1 protein expression, six presented nodal metastases (12 per cent). Cyclin D1 protein expression was found in five per cent of the specimens of normal mucosa, eight per cent of thosewith low-grade dysplasia and 20 per cent of those with high-grade dysplasia. A statistically significant association was found between cyclin D1 expression and the supraglottic site (p<0.05), tumour extension (p<0.001), the presence of lymph node metastases (p<0.001), and advanced clinical stage (p<0.001). Cyclin D2 expression analysis is an important tool in the selection of LSCC patients with an aggressive clinical course.

Keywords

Carcinomasquamous cellLarynxTumour markersbiological
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 111 , Issue 7 , July 1997 , pp. 622 - 626
Copyright
Copyright © JLO (1984) Limited 1997

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