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Light and confocal microscopic observations of adult Schistosoma mansoni from mice fed on a high-fat diet

Published online by Cambridge University Press:  01 December 2007

Renata H. Neves ,
Alba C.M.B. Alencar ,
Marcia B. Águila ,
Carlos A. Mandarim-de-Lacerda ,
José R. Machado- Silva  and
Delir C. Gomes
Renata H. Neves
Affiliation:
Laboratory of Helminthology Romero Lascasas Porto, Department of Microbiology, Immunology and Parasitology, Faculty of Medical Sciences, Biomedical Center, Rua Prof. Manuel de Abreu 444/50 andar, Vila Isabel, 20511-070, Rio de Janeiro, Brazil Laboratory of Helminth Parasites of Vertebrates, Department of Helminthology, Oswaldo Cruz Institute, Av. Brasil 4365, Manguinhos, 21045-900, Rio de Janeiro, Brazil
Alba C.M.B. Alencar
Affiliation:
Laboratory of Helminthology Romero Lascasas Porto, Department of Microbiology, Immunology and Parasitology, Faculty of Medical Sciences, Biomedical Center, Rua Prof. Manuel de Abreu 444/50 andar, Vila Isabel, 20511-070, Rio de Janeiro, Brazil
Marcia B. Águila
Affiliation:
Laboratory of Morphometry and Cardiovascular Morphology, Institute of Biology, Biomedical Center, State University of Rio de Janeiro, Av. 28 de Setembro 87, Vila Isabel, 20551-030, Rio de Janeiro, Brazil
Carlos A. Mandarim-de-Lacerda
Affiliation:
Laboratory of Morphometry and Cardiovascular Morphology, Institute of Biology, Biomedical Center, State University of Rio de Janeiro, Av. 28 de Setembro 87, Vila Isabel, 20551-030, Rio de Janeiro, Brazil
José R. Machado- Silva
Affiliation:
Laboratory of Helminthology Romero Lascasas Porto, Department of Microbiology, Immunology and Parasitology, Faculty of Medical Sciences, Biomedical Center, Rua Prof. Manuel de Abreu 444/50 andar, Vila Isabel, 20511-070, Rio de Janeiro, Brazil
Delir C. Gomes*
Affiliation:
Laboratory of Helminth Parasites of Vertebrates, Department of Helminthology, Oswaldo Cruz Institute, Av. Brasil 4365, Manguinhos, 21045-900, Rio de Janeiro, Brazil
*
*Tel/Fax: +55 21 25984363, E-mail: dcgomes@ioc.fiocruz.br
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Abstract

The morphological aspects of Schistosoma mansoni adult worms recovered from albino mice fed on a cholesterol-rich diet compared to mice fed on a standard chow were investigated. After feeding on their respective diets for over a period of 5 months, mice were subcutaneously infected with c. 50 S. mansoni cercariae/mouse. Blood samples were obtained 1 day prior to experimental infections and 63 days later, when mice were euthanized by jugular section (hypovolaemic shock). Total cholesterol (TC) levels were determined. Recovered worms were stained with hydrochloric carmine, and preserved as whole-mounts for examination by bright-field and laser confocal microscopy. The infected mice fed on high-fat chow showed higher levels of serum lipoproteins than the infected mice fed on standard chow, except for very-low-density lipoprotein cholesterol (VLDL-c) and triglycerides (TG). In this experiment, worms from mice fed on a high-fat chow showed a greater percentage of morphological differentiation regarding supernumerary testes, seminal vesicle, and seminal receptacle. In mice of this group, the rate of oocyte laying in the ovary was much higher than in control females. The present results suggest that cholesterol could be actively involved in the modulation of cell signalling and reproduction, because the lobes contained fully developed oocytes in variable amounts, different from control males. The data presented here are the first to report the role of a cholesterol-rich diet affecting the development of S. mansoni worms.

Type
Research Papers
Information
Journal of Helminthology , Volume 81 , Issue 4 , December 2007 , pp. 361 - 368
Copyright
Copyright © Cambridge University Press 2007

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