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European Archives of Oto-Rhino-Laryngology

13-11-2024 | Lichen Planus | Head and Neck

Quantitative proteogenomic characterization in MUC1 and MUC4 in oral squamous cell carcinoma, oral potentially malignant disorders, and normal oral mucosa in carcinogenesis

Authors: Sharon John, Kriti Sharma, Priyanka Singh, Shaleen Chandra, Geeta Singh, Shalini Gupta

Published in: European Archives of Oto-Rhino-Laryngology

Abstract

Introduction

Mucins are glycoproteins with a significant molecular weight that have a diverse range of biological functions. MUC1 & MUC4 are transmembrane mucin family members that are expressed in airway epithelial cells and body fluids. It is expressed excessively in numerous carcinomas in addition to their altered expression. A thorough review of the literature reveals very limited research about MUC1 & 4 expressions in oral malignancies and oral premalignant disorders. The aim of the research is to assess the pattern and quantity of gene expression in MUC1 & MUC4 individually in various grades of oral squamous cell carcinoma (OSCC), oral premalignant disorders (OPMDs)- oral epithelial dysplasia (OED), oral submucous fibrosis (OSF), oral lichen planus (OLP), and compared with normal oral mucosa (NOM).

Materials and methods

Immunohistochemistry and qRTPCR evaluation of MUC1 & 4 on sixty-three cases of OSCC, OPMD, and NOM was accomplished. The one-way ANOVA test and Chi-square test were done for statistical analysis.

Results

The overall immunoexpression of MUC1 & 4 increased significantly from NOM to OPMDs to OSCC. In the subgrades of OSCC and OPMDs, the staining intensity varied. For MUC4, the intensity was strongest in well-differentiated grades and MUC1, exhibiting a higher expression in poorly differentiated grades of OSCC and OPMDs.

Conclusion

The study's results indicate that MUC1 & 4 individually are crucial for monitoring OSCC and OPMD pathogenesis as the former gives an idea of highly undifferentiated grades while the latter indicated more differentiated tumors and perhaps a better prognosis. Therefore, the two can be useful tumor markers for determining the severity and eliminating it in its early phases.

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Title: Quantitative proteogenomic characterization in MUC1 and MUC4 in oral squamous cell carcinoma, oral potentially malignant disorders, and normal oral mucosa in carcinogenesis
Authors: Sharon John
Kriti Sharma
Priyanka Singh
Shaleen Chandra
Geeta Singh
Shalini Gupta
Publication date: 13-11-2024
Publisher: Springer Berlin Heidelberg
Keyword: Lichen Planus
Published in: European Archives of Oto-Rhino-Laryngology
Print ISSN: 0937-4477
Electronic ISSN: 1434-4726
DOI: https://doi.org/10.1007/s00405-024-09056-3

Podcast | Sexual health in older age

Springer Medicine

Quantitative proteogenomic characterization in MUC1 and MUC4 in oral squamous cell carcinoma, oral potentially malignant disorders, and normal oral mucosa in carcinogenesis

Abstract

Introduction

Materials and methods

Results

Conclusion

Podcast | Sexual health in older age

Springer Medicine

Abstract

Introduction

Materials and methods

Results

Conclusion

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