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Published in: Brain Structure and Function 5/2024

04-05-2024 | Intense Pulsed Light | Original Article

Cell-specific localization of β-synuclein in the mouse retina

Authors: Wenhui Zhong, Qingwen Yang, Fenglan Wang, Xin Lin, Zhongqun Chen, Jing Xue, Wenna Zhao, Xiaoqing Liu, Bilin Rao, Jun Zhang

Published in: Brain Structure and Function | Issue 5/2024

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Abstract

β-synuclein, a member of the synuclein family, is frequently co-expressed with α-synuclein in the neural system, where it serves to inhibit abnormal aggregation of α-synuclein in neurodegenerative diseases. Beyond its role in pathological conditions, β-synuclein plays various functions independently of α-synuclein. In our investigation, we discovered a broader expression of β-synuclein in the mouse retina compared to α-synuclein. This widespread pattern implies its potential significance in the retina. Through detailed examination via light- and electron-microscopic immunocytochemistry, we identified β-synuclein expression from the inner segment (IS) and outer segment (OS) of photoreceptor cells to the ganglion cell layer (GCL). Our findings unveiled unique features, including β-synuclein immunoreactive IS and OS of cones, higher expression in cone pedicles than in rod spherules, absence in horizontal cells, limited expression in cone bipolar dendrites and somas, higher expression in cone bipolar terminals, presence in most amacrine cells, and expression in almost majority of somas in GCL with an absence in intrinsically photosensitive retinal ganglion cell (ipRGCs) processes. Notably, all cholinergic amacrine cells express high β- but not α-synuclein, while dopaminergic amacrine cells express α-synuclein exclusively. These distinctive expression patterns offer valuable insights for further exploration into the functions of β-synuclein and its potential role in synuclein pathology within the retina.
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Metadata
Title
Cell-specific localization of β-synuclein in the mouse retina
Authors
Wenhui Zhong
Qingwen Yang
Fenglan Wang
Xin Lin
Zhongqun Chen
Jing Xue
Wenna Zhao
Xiaoqing Liu
Bilin Rao
Jun Zhang
Publication date
04-05-2024
Publisher
Springer Berlin Heidelberg
Published in
Brain Structure and Function / Issue 5/2024
Print ISSN: 1863-2653
Electronic ISSN: 1863-2661
DOI
https://doi.org/10.1007/s00429-024-02799-z

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