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Journal of Natural Medicines
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06-09-2022 | Note

HPLC analysis of ammonium glycyrrhizate listed in the European, United States, and Japanese Pharmacopoeias under reported and modified conditions: revision of the peak assignment for 18α-glycyrrhizin in the European and United States Pharmacopoeias

Authors: Kazuaki Kamata, Jin Tatsuzaki, Tsutomu Ishikawa, Ryoko Arai, Takashi Hakamatsuka, Nahoko Uchiyama

Published in: Journal of Natural Medicines | Issue 1/2023

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Abstract

We examined ammonium glycyrrhizate listed in the monographs of the European Pharmacopoeia (EP) and United States Pharmacopoeia (USP) as well as in the reagents and solutions used in the general test of the Japanese Pharmacopoeia by performing HPLC on their sample standards or reference reagents under reported and modified conditions. Comparative experiments involving five authentic samples, namely, 18β-glycyrrhizin (1), 18α-glycyrrhizin (2), licorice–saponin G2 (3), licorice–saponin H2 (4), and galacturonic acid-replaced glycyrrhizin (the 4″-epimer of 18β-glycyrrhizin) (5), led us to propose the revision of the peak assignment of 18α-glycyrrhizin (2) and postscript a possible co-existence of galacturonic acid-replaced glycyrrhizin (5) as a hidden component in the EP and USP. We also proposed that the α-configuration used in the nomenclature of the glycosidic bond between aglycone and the sugar units of ammonium glycyrrhizate and impurities in the EP and USP should be revised to the β-configuration.

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Metadata
Title
HPLC analysis of ammonium glycyrrhizate listed in the European, United States, and Japanese Pharmacopoeias under reported and modified conditions: revision of the peak assignment for 18α-glycyrrhizin in the European and United States Pharmacopoeias
Authors
Kazuaki Kamata
Jin Tatsuzaki
Tsutomu Ishikawa
Ryoko Arai
Takashi Hakamatsuka
Nahoko Uchiyama
Publication date
06-09-2022
Publisher
Springer Nature Singapore
Published in
Journal of Natural Medicines / Issue 1/2023
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI
https://doi.org/10.1007/s11418-022-01649-8

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