Skip to main content
Top

25-04-2024 | Fibromyalgia | Original Article

Diosmetin attenuates fibromyalgia-like symptoms in a reserpine-induced model in mice

Authors: Lara Panazzolo Marquezin, Maria Fernanda Pessano Fialho, Amanda Favarin, Jéssica Dotto de Lara, Micheli Mainardi Pillat, Denis Broock Rosemberg, Sara Marchesan Oliveira

Published in: Inflammopharmacology

Login to get access

Abstract

Fibromyalgia is a potentially disabling idiopathic disease characterized by widespread chronic pain associated with comorbidities such as fatigue, anxiety, and depression. Current therapeutic approaches present adverse effects that limit adherence to therapy. Diosmetin, an aglycone of the flavonoid glycoside diosmin found in citrus fruits and the leaves of Olea europaea L., has antinociceptive, anti-inflammatory, and antioxidant properties. Here, we investigated the effect of diosmetin on nociceptive behaviors and comorbidities in an experimental fibromyalgia model induced by reserpine in mice. To induce the experimental fibromyalgia model, a protocol of subcutaneous injections of reserpine (1 mg/kg) was used once a day for three consecutive days in adult male Swiss mice. Mice received oral diosmetin on the fourth day after the first reserpine injection. Nociceptive (mechanical allodynia, muscle strength, and thermal hyperalgesia) and comorbid (depressive-like and anxiety behavior) parameters were evaluated. Potential adverse effects associated with diosmetin plus reserpine (locomotor alteration, cataleptic behavior, and body weight and temperature changes) were also evaluated. Oral diosmetin (0.015–1.5 mg/kg) reduced the mechanical allodynia, thermal hyperalgesia, and loss of muscle strength induced by reserpine. Diosmetin (0.15 mg/kg) also attenuated depressive-like and anxiety behaviors without causing locomotor alteration, cataleptic behavior, and alteration in weight and body temperature of mice. Overall, diosmetin can be an effective and safe therapeutic alternative to treat fibromyalgia symptoms, such as pain, depression and anxiety.
Literature
go back to reference De la Luz-Cuellar YE, Rodríguez-Palma EJ, Franco-Enzástiga Ú et al (2019) Blockade of spinal receptors differentially reduces reserpine-induced fibromyalgia-type pain in female rats. Eur J Pharmacol 858:1724443CrossRef De la Luz-Cuellar YE, Rodríguez-Palma EJ, Franco-Enzástiga Ú et al (2019) Blockade of spinal receptors differentially reduces reserpine-induced fibromyalgia-type pain in female rats. Eur J Pharmacol 858:1724443CrossRef
go back to reference Liao W, Ning Z, Chen L et al (2014) Intracellular antioxidant detoxifying effects of diosmetin on 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress through inhibition of reactive oxygen species generation. J Agric Food Chem 62:8648–8654. https://doi.org/10.1021/jf502359xCrossRefPubMed Liao W, Ning Z, Chen L et al (2014) Intracellular antioxidant detoxifying effects of diosmetin on 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress through inhibition of reactive oxygen species generation. J Agric Food Chem 62:8648–8654. https://​doi.​org/​10.​1021/​jf502359xCrossRefPubMed
go back to reference Yang Y, Gong X, Huang L et al (2017) Diosmetin exerts anti-oxidative, anti-inflammatory and anti-apoptotic effects to protect against endotoxin-induced acute hepatic failure in mice. Oncotarget 8:30723CrossRefPubMedPubMedCentral Yang Y, Gong X, Huang L et al (2017) Diosmetin exerts anti-oxidative, anti-inflammatory and anti-apoptotic effects to protect against endotoxin-induced acute hepatic failure in mice. Oncotarget 8:30723CrossRefPubMedPubMedCentral
go back to reference Yao X, Li L, Kandhare A et al (2019) Attenuation of reserpine-induced fibromyalgia via ROS and serotonergic pathway modulation by fisetin a plant flavonoid polyphenol. Exp Ther Med 1343:1355 Yao X, Li L, Kandhare A et al (2019) Attenuation of reserpine-induced fibromyalgia via ROS and serotonergic pathway modulation by fisetin a plant flavonoid polyphenol. Exp Ther Med 1343:1355
Metadata
Title
Diosmetin attenuates fibromyalgia-like symptoms in a reserpine-induced model in mice
Authors
Lara Panazzolo Marquezin
Maria Fernanda Pessano Fialho
Amanda Favarin
Jéssica Dotto de Lara
Micheli Mainardi Pillat
Denis Broock Rosemberg
Sara Marchesan Oliveira
Publication date
25-04-2024
Publisher
Springer International Publishing
Published in
Inflammopharmacology
Print ISSN: 0925-4692
Electronic ISSN: 1568-5608
DOI
https://doi.org/10.1007/s10787-024-01473-4