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Aesthetic Plastic Surgery
Published in: Aesthetic Plastic Surgery 3/2022

21-10-2021 | Fat Grafting | Original Article

“Fasting: An Effective Preconditioning Method to Increase Fat Graft Survival”

Authors: Han Gyu Cha, Dong Gyu Kim, Jiyeon Chang, Yuri Song, Seongfeel Jeong, Seung Min Nam, Syeo Young Wee, Kae Won Cho, Chang Yong Choi

Published in: Aesthetic Plastic Surgery | Issue 3/2022

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Abstract

Background

Most preconditioning techniques before fat grafting require external manipulation. Since nutrition is the main factor maintaining the balance of lipogenesis and lipolysis, we hypothesized that fasting before undergoing autologous fat grafting may increase lipolysis and reduce adipocyte size, thereby improving the fat graft survival rate.

Methods

C57BL/6 mice were divided into 24 h starved or fed groups. Adipose tissue lipolysis, adipogenesis, and angiogenesis-related gene expression, in fat from both groups, were analyzed. The volume and weight of the grafted fat at 4–8 weeks postoperatively were measured using micro-computed tomography. Immunohistochemistry staining and mRNA expression analysis were also performed to evaluate the effect of fasting on fat graft survival.

Results

Fasting decreased adipocyte size by inducing adipose tissue lipolysis. Adipogenesis-related genes were remarkably downregulated while lipolysis-related genes and angiogenesis inducer genes were significantly upregulated in the starved adipose tissue. The mice grafted with the fat from the 24 h starved group had approximately 20% larger volumes and considerably heavier weights than those from the fed group. Increased viable adipocytes and vessels, and reduced macrophages in the fat grafts obtained from the 24 h starved group were also observed.

Conclusions

Fasting for 24 h before harvesting fat increased the retention volume of fat graft by increasing angiogenesis via VEGF induction. Therefore, fasting would be a novel and reliable preconditioning strategy to improve graft survival in autologous fat grafting.

No Level Assigned

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Metadata
Title
“Fasting: An Effective Preconditioning Method to Increase Fat Graft Survival”
Authors
Han Gyu Cha
Dong Gyu Kim
Jiyeon Chang
Yuri Song
Seongfeel Jeong
Seung Min Nam
Syeo Young Wee
Kae Won Cho
Chang Yong Choi
Publication date
21-10-2021
Publisher
Springer US
Published in
Aesthetic Plastic Surgery / Issue 3/2022
Print ISSN: 0364-216X
Electronic ISSN: 1432-5241
DOI
https://doi.org/10.1007/s00266-021-02630-8

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