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Published in: International Urogynecology Journal 5/2024

25-04-2024 | Epstein-Barr Virus | Original Article

PD-L1 Expression in Nonbacterial Chronic Cystitis and Bladder Cancer

Authors: Inga Kosova, Vagan Barsegian, Lyudmila Gundorova, Dmitriy Kolbasov

Published in: International Urogynecology Journal | Issue 5/2024

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Abstract

Introduction and hypothesis

The objective was to assess PD-L1 expression in nonbacterial chronic cystitis (NCC) and bladder cancer (BC).

Methods

The present study included 20 NCC and 20 BC patients. The degree of inflammation of the bladder wall was assessed on slides stained with H&E. Viral pathogens (herpes simplex virus, Epstein–Barr virus, cytomegalovirus, and high-risk HPVs) were detected using real-time polymerase chain reaction analyses of the bladder specimens. Immunohistochemistry was performed to assess the PD-L1 expression in bladder tissue.

Results

Expression of PD-L1 was detected in 40% of NCC patients and 85% of BC patients. Viral pathogens were found in 50% of NCC patients and 60% of BC patients, with EBV being the most common. In NCC patients the immune cell score correlated strongly with the degree of inflammatory infiltration of the bladder wall (r = 0.867, p < 0.001), the presence of lymphoid aggregates in the submucosa (r = 0.804, p < 0.001), koilocytosis (r = 0.620, p = 0.004), and the presence of viral pathogens (r = 0.784, p < 0.001). In BC patients the immune cell score correlated with the degree of inflammatory infiltration of the bladder wall (r = 0.534, p = 0.015) and the presence of viral pathogens (r = 0.626, p = 0.003), but not with the presence of lymphoid aggregates in the submucosa (r = 0.083, p = 0.729), and koilocytosis (r = 0.366, p = 0.112).

Conclusions

Expression of PD-L1 was detected in a cohort of NCC patients, although the PD-L1 positivity rate was lower than that in BC. Our results demonstrate that the degree of PD-L1 expression in bladder tissue is associated with the presence of viral infections and with the degree of inflammatory infiltration of the bladder wall in both NCC and BC.
Literature
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go back to reference Barsegian VA, Kosova IV. Role of the lower urinary tract viral infections in the development of female micturition disorders. Urologiia. 2022;5:117–22. Barsegian VA, Kosova IV. Role of the lower urinary tract viral infections in the development of female micturition disorders. Urologiia. 2022;5:117–22.
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go back to reference Nechifor-Boilă IA, Loghin A, Nechifor-Boilă A, et al. PD-L1 expression in muscle invasive urothelial carcinomas as assessed via immunohistochemistry: correlations with specific clinical and pathological features, with emphasis on prognosis after radical cystectomy. Life (Basel). 2021;11(5):404. https://doi.org/10.3390/life11050404.CrossRefPubMed Nechifor-Boilă IA, Loghin A, Nechifor-Boilă A, et al. PD-L1 expression in muscle invasive urothelial carcinomas as assessed via immunohistochemistry: correlations with specific clinical and pathological features, with emphasis on prognosis after radical cystectomy. Life (Basel). 2021;11(5):404. https://​doi.​org/​10.​3390/​life11050404.CrossRefPubMed
Metadata
Title
PD-L1 Expression in Nonbacterial Chronic Cystitis and Bladder Cancer
Authors
Inga Kosova
Vagan Barsegian
Lyudmila Gundorova
Dmitriy Kolbasov
Publication date
25-04-2024
Publisher
Springer International Publishing
Published in
International Urogynecology Journal / Issue 5/2024
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI
https://doi.org/10.1007/s00192-024-05782-8

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