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BMC Oral Health
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Open Access 01-12-2024 | Research

Effect of oxyresveratrol under in vitro lipopolysaccharide-induced periodontitis environment

Authors: Ju Ri Ye, Seung Hwan Park, Sang Wook Kang, Kyu Hwan Kwack, Yong Kwon Chae, Hyo-Seol Lee, Sung Chul Choi, Ok Hyung Nam

Published in: BMC Oral Health | Issue 1/2024

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Abstract

Background

Oxyresveratrol is the main constituent of mulberries and has many bioactive properties beneficial to human health. The purpose of this study was to assess the anti-inflammatory effects of oxyresveratrol on in vitro periodontitis model.

Methods

Human periodontal ligament cells were treated with oxyresveratrol (0, 10, and 20 µg/mL) for 72 h. Cell viability and flow cytometry assays were performed. To investigate anti-inflammatory effect of oxyresveratrol on periodontal inflammation, nitric oxide production under lipopolysaccharide stimulation was assessed. Next, expression of biomarkers associated periodontal inflammation was evaluated. Scratch wound assay was performed to evaluate cell migration/proliferation potential of oxyresveratrol under lipopolysaccharide stimulation.

Results

Periodontal ligament cell toxicity was not observed in oxyresveratrol treatment. Oxyresveratrol treatment significantly inhibited nitric oxide production and reduced MMP-2, MMP-9, TNF-α, IL-6, and IL-8 expressions after lipopolysaccharide stimulation. Regarding cell migration/proliferation, open wound area in oxyresveratrol (33.28 ± 6.80%) was the lowest (p < 0.05).

Conclusions

Within the limits of this study, oxyresveratrol inhibited lipopolysaccharide-induced inflammation in periodontal ligament cells and promoted periodontal ligament cell migration/proliferation. These findings suggest that oxyresveratrol could be valuable for the management of periodontal diseases.
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Metadata
Title
Effect of oxyresveratrol under in vitro lipopolysaccharide-induced periodontitis environment
Authors
Ju Ri Ye
Seung Hwan Park
Sang Wook Kang
Kyu Hwan Kwack
Yong Kwon Chae
Hyo-Seol Lee
Sung Chul Choi
Ok Hyung Nam
Publication date
01-12-2024
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2024
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-024-05128-2

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