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Direct oral anticoagulants do not affect miR-27a-3p expression, a regulator of coagulation cascade, in atrial fibrillation patients

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Published in:

Open Access 21-04-2025 | Direct Oral Anticoagulant

Direct oral anticoagulants do not affect miR-27a-3p expression, a regulator of coagulation cascade, in atrial fibrillation patients

Authors: Georgia Ragia, Myria Pallikarou, Chrysoula Michou, Thomas Thomopoulos, Georgios Chalikias, Athanasios Trikas, Dimitrios N. Tziakas, Vangelis G. Manolopoulos

Published in: Journal of Thrombosis and Thrombolysis | Issue 5/2025

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Abstract

miR-27a-3p targets several proteins on the coagulation cascade. The potential effect of direct oral anticoagulants (DOACs) treatment on miR-27a-3p expression and their broader regulative effect on anticoagulation is unknown. Fifty-nine atrial fibrillation patients treated with rivaroxaban (n = 19), apixaban (n = 27) or dabigatran (n = 13), were included in the study. miR-27a-3p expression was analyzed at baseline and after 7 days of DOAC therapy by using a predesigned TaqMan assay. Relative quantitation of miR-27a-3p expression was calculated and compared in pooled population and in different sample groups. DOAC therapy did not alter miR-27a-3p expression (0.80 fold-change, p = 0.486, pooled population; 0.839 fold-change, p = 0.706, rivaroxaban; 0.921 fold-change, p = 0.800, apixaban; 0.733 fold-change, p = 0.540, dabigatran). miR-27a-3p expression did not differ between controls and bleeding cases (0.833 fold-change, p = 0.588, baseline). Female patients had a trend towards increased baseline expression (1.564 fold-change, p = 0.177) and reduced expression after DOAC treatment (0.683 fold-change, p = 0.243) compared to male patients. Despite the regulatory role of miR-27a-3p on coagulation cascade, treatment with DOACs did not alter its expression. However, additional studies in different ethnic groups are necessary to fully elucidate the effect, if any, of DOACs on miR-27a-3p expression.

Graphical Abstract

Direct oral anticoagulant effect on miR-27a-3p expression in atrial fibrillation. Both miR-27a-3p and direct oral anticoagulants (DOACs) act on the coagulation cascade. Following over time changes in miR-27a-3p expression in DOAC treated atrial fibrillation patients, we have shown that DOAC treatment does not alter its expression
Literature
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Metadata
Title
Direct oral anticoagulants do not affect miR-27a-3p expression, a regulator of coagulation cascade, in atrial fibrillation patients
Authors
Georgia Ragia
Myria Pallikarou
Chrysoula Michou
Thomas Thomopoulos
Georgios Chalikias
Athanasios Trikas
Dimitrios N. Tziakas
Vangelis G. Manolopoulos
Publication date
21-04-2025
Publisher
Springer US
Published in
Journal of Thrombosis and Thrombolysis / Issue 5/2025
Print ISSN: 0929-5305
Electronic ISSN: 1573-742X
DOI
https://doi.org/10.1007/s11239-025-03102-5