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Published in: Indian Journal of Otolaryngology and Head & Neck Surgery 1/2023

21-01-2023 | Original Article

Differential Angiogenic Induction Impacts Nasal Polyp Tissue Growth

Authors: Hallyson Andre Nascimento de Góes, Masih Sarafan, Jônatas Bussador do Amaral, Joyce dos Anjos Almeida, Richard Louis Voegels, Leandro Ticlia de la Cruz, Andrew Thamboo, Lígia Ferreira Gomes, Rogério Pezato

Published in: Indian Journal of Otolaryngology and Head & Neck Surgery | Special Issue 1/2023

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Abstract

In chronic rhinosinusitis with nasal polyps, inflammatory edema drives tissue remodeling favoring anomalous growth of the nasal mucosa, but a proangiogenic contribution of nasal polyp in support of tissue growth is still controversial. The chorioallantoic membrane of chicken embryo model was employed to address the potentiality of nasal tissue fragments to modulate angiogenesis. Fifty-seven fertilized eggs were implanted with polyp or healthy nasal mucosa tissue or were kept as non-implanted controls. The embryos’ size, length, and development stage, and chorioallantoic membrane vasculature morphology were evaluated after 48 h. Quantitative computer vision techniques applied to digital chorioallantoic membrane images automatically calculated the branching index as the ratio between the areas of the convex polygon surrounding the vascular tree and the vessels’ area. Ethics approval and consent to participate: the study was approved by the Human Research Ethics Committee of the Federal University of São Paulo (CAAE number: 80763117.1.0000.5505) and by the Animal Research Ethics Committee of University of São Paulo (nº CEUA 602–2019). Mucosal, but not polyp tissue implants, hampered embryo development and induced underdeveloped chorioallantoic membranes with anastomosed, interrupted, and regressive vessels. Vessels’ areas and branching indexes were higher among the chorioallantoic membranes with polyp implants and controls than among those with healthy mucosa implants. Nasal polyp presents differential angiogenic induction that impacts tissue growth.
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Metadata
Title
Differential Angiogenic Induction Impacts Nasal Polyp Tissue Growth
Authors
Hallyson Andre Nascimento de Góes
Masih Sarafan
Jônatas Bussador do Amaral
Joyce dos Anjos Almeida
Richard Louis Voegels
Leandro Ticlia de la Cruz
Andrew Thamboo
Lígia Ferreira Gomes
Rogério Pezato
Publication date
21-01-2023
Publisher
Springer India
Published in
Indian Journal of Otolaryngology and Head & Neck Surgery / Issue Special Issue 1/2023
Print ISSN: 2231-3796
Electronic ISSN: 0973-7707
DOI
https://doi.org/10.1007/s12070-023-03469-y

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