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01-11-2024 | Annotated Sequence Record

Complete genome sequence of a novel mitovirus identified in the phytopathogenic fungus Alternaria tenuissima

Authors: Tongyu Mu, Zhonglei Wang, Zhijun Liu, Xuehong Wu

Published in: Archives of Virology | Issue 11/2024

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Abstract

In this study, a novel positive-sense single-stranded RNA (+ ssRNA) mycovirus, Alternaria tenuissima mitovirus 1 (AtMV1), was identified in Alternaria tenuissima strain YQ-2-1, a phytopathogenic fungus causing leaf blight on muskmelon. The genome of AtMV1 is a single RNA molecule that is 3013 nt in length with an A + U content of 66.58% and contains a single open reading frame (ORF) using the fungal mitochondrial genetic code. The ORF was predicted to encode a 313-amino-acid RNA-dependent RNA polymerase (RdRp) with a molecular mass of 35.48 kDa, which contains six conserved motifs with the highly conserved GDD tripeptide in motif IV. The 5ʹ and 3ʹ untranslated regions were predicted to fold into stem-loop and panhandle secondary structures. The results of a BLASTp search revealed that the amino acid (aa) sequence of RdRp of AtMV1 shared the highest sequence similarity (51.04% identity) with that of Sichuan mito-like virus 30, a member of the genus Duamitovirus within the family Mitoviridae. Phylogenetic analysis based on the aa sequence of the RdRp suggested that AtMV1 is a novel member of the genus Duamitovirus. To our knowledge, this is the first report of the complete genome sequence of a new mitovirus infecting A. tenuissima.
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Metadata
Title
Complete genome sequence of a novel mitovirus identified in the phytopathogenic fungus Alternaria tenuissima
Authors
Tongyu Mu
Zhonglei Wang
Zhijun Liu
Xuehong Wu
Publication date
01-11-2024
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 11/2024
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-024-06145-w

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