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Open Access 01-12-2024 | Colorectal Cancer | Research

Concordance between immunohistochemistry and MSI analysis for detection of MMR/MSI status in colorectal cancer patients

Authors: Muhammad Ishaque Faizee, NorLelawati A. Talib, Asmah Hanim Bt. Hamdan, Nor Zamzila Bt. Abdullah, Bilal Ahmad Rahimi, Ahmed Maseh Haidary, Ramin Saadaat, Ahmed Nasir Hanifi

Published in: Diagnostic Pathology | Issue 1/2024

Abstract

Background

Recently, screening of colorectal cancer (CRC) patients for mismatch repair/microsatellite instability (MMR/MSI) status is widely practiced due to its potential predictive and prognostic roles and a screening tool to reveal Lynch Syndrome (LS). The purpose of the study was to evaluate concordance between immunohistochemistry (IHC) and MSI analysis methods for detection of MMR/MSI status in colorectal cancer patients in Kuantan, Pahang.

Methods

Fifty selected CRC cases of deficient mismatch repair (dMMR) and proficient mismatch repair (pMMR) which were identified immunohistochemically in the previous study were subjected to MSI analysis. MSI Analysis System 1.2 (Promega) was utilized.

Results

The results of MSI analysis method showed MSI-High: 26% (13/50), MSI-Low: 6% (3/50), and Microsatellite Stable: 68% (34/50). The concordance was perfect (0.896, Kappa value) between MSI analysis and IHC methods for the assessment of MMR/MSI status in CRC patients. The discordance was only 4% (2/50). MSI analysis identified all dMMR cases determined by IHC except one case. The obtained frequency of dMMR and pMMR patients was 11.4% (14/123) and 88.6% (109/123) by IHC method, respectively.

Conclusion

Our findings support the universal practice of evaluating the MMR/MSI status in all newly diagnosed CRC patients. Based on the perfect concordance of two methods, the method of choice is based on the availability of expertise and equipments. IHC is highly appreciable method due to its feasibility and reproducibility.

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Title: Concordance between immunohistochemistry and MSI analysis for detection of MMR/MSI status in colorectal cancer patients
Authors: Muhammad Ishaque Faizee
NorLelawati A. Talib
Asmah Hanim Bt. Hamdan
Nor Zamzila Bt. Abdullah
Bilal Ahmad Rahimi
Ahmed Maseh Haidary
Ramin Saadaat
Ahmed Nasir Hanifi
Publication date: 01-12-2024
Publisher: BioMed Central
Keywords: Colorectal Cancer
Colorectal Cancer
Published in: Diagnostic Pathology / Issue 1/2024
Electronic ISSN: 1746-1596
DOI: https://doi.org/10.1186/s13000-024-01571-5

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