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25-04-2024 | Cefpodoxime | Original Article

MAST® D72C test: a novel option for ESBL, AmpC and carbapenemase detection

Authors: C. C. Noubam-Tchatat, E. Maurin, S. Proust, R. Beyrouthy, R. Bonnet, Frederic Robin

Published in: European Journal of Clinical Microbiology & Infectious Diseases

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Abstract

Purpose

The MAST® D72C test is a phenotypical test which can detect ESBL and AmpC production in Enterobacterales. It can also identify the suspected presence of carbapenemase. The aim of the present study was to assess the sensitivity and specificity of this test and to discuss its usefulness in laboratories, especially those that use only an automated AST system.

Method

The performance of the MAST® D72C test was assessed against a collection of 119 non-redundant Enterobacterales isolates characterized for their content in β-lactamases, and compared with that of the reference double disk synergy test. β-lactamase content was established from phenotypic and genotypic analyses to collect a broad diversity of resistance mechanisms and bacterial strains, including 30 ESBL-producing strains, 32 strains overproducing chromosomal AmpC, 10 strains producing plasmid-encoded AmpC, 12 carbapenemase-producing strains, 13 strains combining the production of several β-lactamases, and 22 strains that produced other β-lactamases.

Results

The sensitivity and specificity for ESBL-detection were comparable with those of the synergy test, 75 versus 72.5%, and 94.9 versus 93.7%, respectively. The sensitivity and specificity for AmpC-detection were 71.7% and 100%, respectively, and sensitivity reached 78.7% if we excluded carbapenem-resistant isolates. Carbapenemase-detection sensitivity was 90%.

Conclusion

These results show that the MAST® D72C test can be a useful tool for the detection of ESBL- and AmpC-production in clinical laboratories.
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Metadata
Title
MAST® D72C test: a novel option for ESBL, AmpC and carbapenemase detection
Authors
C. C. Noubam-Tchatat
E. Maurin
S. Proust
R. Beyrouthy
R. Bonnet
Frederic Robin
Publication date
25-04-2024
Publisher
Springer Berlin Heidelberg
Published in
European Journal of Clinical Microbiology & Infectious Diseases
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI
https://doi.org/10.1007/s10096-024-04829-4
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