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08-06-2024 | Cataract | Basic Science

Proteomics identifies hypothermia induced adiponectin protects corneal endothelial cells via AMPK mediated autophagy in phacoemulsification

Authors: Yanyi Chen, Kewei Li, Rongxi Huang, Liang Xiong, Ruonan Li, Lu Jiang, Yan Xun, Wenjuan Wan, Ke Hu

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology

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Abstract

Aim

To explore the molecular mechanism underlying the protective effect of hypothermic perfusion on the corneal endothelium during phacoemulsification.

Methods

Phacoemulsification was performed on New Zealand white rabbits. Perfusate at different temperatures was used during the operation, and the aqueous humor was collected for proteomic sequencing after the operation. Corneal endothelial cell injury was simulated by a corneal endothelial cell oxygen–glucose deprivation/reoxygenation (OGD/R) model in vitro. Flow cytometry and evaluation of fluorescent LC3B puncta were used to detect apoptosis and autophagy, and western blotting was used to detect protein expression.

Results

A total of 381 differentially expressed proteins were identified between the two groups. In vitro, 4 ℃ hypothermia significantly reduced apoptosis and promoted autophagy. Apoptosis increased after autophagy was inhibited by 3-Methyladenine (3-MA). Furthermore, adiponectin (ADIPOQ) knockdown inhibited phospho-AMPK and blocked the protective effect of hypothermia on corneal endothelial cells.

Conclusions

We investigated the differential expression of proteins between the hypothermia group and normothermia group by proteomics. Moreover, hypothermia-induced ADIPOQ can reduce apoptosis by promoting AMPK-mediated autophagy.
Literature
Metadata
Title
Proteomics identifies hypothermia induced adiponectin protects corneal endothelial cells via AMPK mediated autophagy in phacoemulsification
Authors
Yanyi Chen
Kewei Li
Rongxi Huang
Liang Xiong
Ruonan Li
Lu Jiang
Yan Xun
Wenjuan Wan
Ke Hu
Publication date
08-06-2024
Publisher
Springer Berlin Heidelberg
Keyword
Cataract
Published in
Graefe's Archive for Clinical and Experimental Ophthalmology
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI
https://doi.org/10.1007/s00417-024-06542-6