Top

European Archives of Paediatric Dentistry

05-03-2025 | Caries | Original Scientific Article

Comparison of retention rates of silver nanoparticles-based fissure sealant and conventional resin-based sealant: a one-year randomized clinical trial

Authors: S. Svetha, K. Bansal, V. P. Mathur, N. Tewari, R. Morankar, M. Kalaivani

Published in: European Archives of Paediatric Dentistry

Abstract

Background

Limited clinical evidence is currently available on the retention rates of the silver-nanoparticles filled pit and fissure sealant in permanent molars.

Aim

To compare the retention rates of a resin-based sealant with silver-nanoparticles with that of a conventional resin-based sealant on permanent molars in children.

Methods

A split-mouth design was used. Six to fourteen years old children (n = 150) reporting to a tertiary care hospital were recruited. A total of 264 pairs of permanent molars were subjected to one of the two interventions-A—Resin-based sealant without silver-nanoparticles (Helioseal F plus sealant); B—resin-based sealant with silver-nanoparticles (e-pit and fissure sealant). Sealants were applied using a standardized technique by a single-blinded operator. Primary outcome was the retention of the sealants and secondary outcomes were the presence or absence of caries, surface roughness, marginal integrity and discoloration of the sealant material. Follow-up evaluations were conducted at 6- and 12-months by two blinded evaluators. Data were analysed using the Chi-square test and p-value less than 0.05 was considered to be significant.

Results

At 12 months, 224 pairs of permanent molars were available for analysis. The test sealant, e-Pit and fissure sealant performed better in terms of retention (68.3% of sealants with silver-nanoparticles retained as compared to 53.6% of resin-based sealants Helioseal F plus; p = 0.001), surface roughness, marginal integrity and sealant discoloration (p < 0.05). The occurrence of caries did not differ significantly between the two groups (p = 0.391).

Conclusion

The study concluded that e-pit and fissure sealant with silver-nanoparticles had a better retention rate as compared to the Helioseal F plus sealant but caries occurrence was not different between the two groups.

Trial registration number

CTRI/2022/05/042622.

Ahovuo-Saloranta A, Forss H, Walsh T, et al. Pit and fissure sealants for preventing dental decay in permanent teeth. Cochrane Database Syst Rev. 2017;7(7):CD001830. https://doi.org/10.1002/14651858.CD001830.pub5.CrossRefPubMed

Attar MH, Abdallah MA, Alharthy HA, El Meligy OA. Effect of bonding agent on retention of different sealants: an in vitro study. J Clin Pediatr Dent. 2021;45(3):177–85. https://doi.org/10.17796/1053-4625-45.3.6.CrossRefPubMed

Bagherian A, Sarraf Shirazi A, Sadeghi R. Adhesive systems under fissure sealants: yes or no? A systematic review and meta-analysis. J Am Dent Assoc. 2016;147(6):446–56. https://doi.org/10.1016/j.adaj.2016.01.014.CrossRefPubMed

Bandi M, Mallineni SK, Nuvvula S. Influence of isolation methods on retention of pit and fissure sealants in young permanent teeth based on Simonsen's criteria: a randomised clinical trial. J Clin Diagn Res. 2021;15(4). https://doi.org/10.7860/JCDR/2021/44929.14734

Bao Z, Sun H, Fan D, Wang X, et al. Shear bond strength and microleakage of pit and fissure sealants placed after saliva-contaminated etched enamel. Coatings. 2022;12(4):441. https://doi.org/10.3390/coatings12040441.CrossRef

Barja-Fidalgo F, Maroun S, de Oliveira BH. Effectiveness of a glass ionomer cement used as a pit and fissure sealant in recently erupted permanent first molars. J Dent Child (Chic). 2009;76(1):34–40.PubMed

Bruna T, Maldonado-Bravo F, Jara P, Caro N. Silver nanoparticles and their antibacterial applications. Int J Mol Sci. 2021;22(13):7202. https://doi.org/10.3390/ijms22137202.CrossRefPubMedPubMedCentral

Deepika B, Vinay C, Uloopi K, Rojaramya KS, et al. Comparison of the retention rates of pit and fissure sealants placed on first permanent molars treated with air abrasion and acid etching: a split-mouth randomised clinical trial. J Clin Diagn Res. 2023. https://doi.org/10.3390/ijms22137202.CrossRef

Dhar V, Chen H. Evaluation of resin based and glass ionomer-based sealants placed with or without tooth preparation-a two-year clinical trial. Pediatr Dent. 2012;34(1):46–50.PubMed

Fei X, Li Y, Weir MD, et al. Novel pit and fissure sealant containing nano-CaF2 and dimethylaminohexadecyl methacrylate with double benefits of fluoride release and antibacterial function. Dent Mater. 2020;36:1241–53. https://doi.org/10.1016/j.dental.2020.05.010.CrossRefPubMed

Gyati O, Jain M, Sogi S, et al. Clinical evaluation of retention of hydrophilic and hydrophobic pit and fissure sealants in permanent first molars: an 18 months follow-up: randomized controlled trial. Int J Clin Pediatr Dent. 2023;16:350–6. https://doi.org/10.5005/jp-journals-10005-2578.CrossRefPubMedPubMedCentral

Handelman SL, Leverett DH, Espeland M, Curzon J. Retention of sealants over carious and sound tooth surfaces. Community Dent Oral Epidemiol. 1987;15:1–5. https://doi.org/10.1111/j.1600-0528.1987.tb00470.x.CrossRefPubMed

Haricharan PB, Barad N, Patil CR, et al. Dawn of a new age fissure sealant? A study evaluating the clinical performance of embrace WetBond and ART sealants: results from a randomized controlled clinical trial. Eur J Dent. 2019;13(4):503–9. https://doi.org/10.1055/s-0039-1696894.CrossRefPubMedPubMedCentral

Hernández-Sierra JF, Ruiz F, Pena DC, et al. The antimicrobial sensitivity of Streptococcus mutans to nanoparticles of silver, zinc oxide, and gold. Nanomedicine. 2008;4(3):237–40. https://doi.org/10.1016/j.nano.2008.04.005.CrossRefPubMed

Huang Y, Li H, Zhu CG, et al. Anti-bacterial and anti-microbial aging effects of resin-based sealant modified by quaternary ammonium monomers. J Dent. 2021;112: 103767. https://doi.org/10.1016/j.jdent.2021.103767.CrossRefPubMed

Khairy SM, El-Tekeya MM, Bakry NS, Aboshelib MN. Evaluation of microleakage of silver nanoparticle-added pit and fissure sealant in permanent teeth (in-vitro study). Alex Dent J. 2020;45:14–8.

Komatsu H, Shimokobe H, Kawakami S, Yoshimura M. Caries-preventive effect of glass ionomer sealant reapplication: study presents three-year results. J Am Dent Assoc. 1994;125:543–9. https://doi.org/10.14219/jada.archive.1994.0082.CrossRefPubMed

Lygidakis NA, Oulis KI. A comparison of Fluroshield with Delton fissure sealant: four year results. Pediatr Dent. 1999;21:429–31.PubMed

Mattar RE, Sulimany AM, Binsaleh SS, et al. Evaluation of fissure sealant retention rates using Isolite in comparison with rubber dam and cotton roll isolation techniques: A randomized clinical trial. Int J Paediatr Dent. 2023;33(1):12–9. https://doi.org/10.1111/ipd.13008.CrossRefPubMed

Morales-Quiroga E, Martínez-Sumarán A, Hernández-Sierra JF, Pozos-Guillén A. Evaluation of marginal seal and microleakage of a sealant modified with silver nanoparticles in primary molars: In vitro study. Odovtos Int J Dent Sci. 2014:107–13.

Nazar H, Mascarenhas AK, Al-Mutwa S, et al. Effectiveness of fissure sealant retention and caries prevention with and without primer and bond. Med Princ Pract. 2013;22(1):12–7. https://doi.org/10.1159/000341155.CrossRefPubMed

Ng TC, Chu CH, Yu OY. A concise review of dental sealants in caries management. Front Oral Health. 2023;4:1180405. https://doi.org/10.3389/froh.2023.1180405.CrossRefPubMedPubMedCentral

Ninawe N, Ullal NA, Khandelwal V. A 1-year clinical evaluation of fissure sealants on permanent first molars. Contemp Clin Dent. 2012;3:54–9. https://doi.org/10.4103/0976-237X.94547.CrossRefPubMedPubMedCentral

Ramesh H, Ashok R, Rajan M, et al. Retention of pit and fissure sealants versus flowable composites in permanent teeth: a systematic review. Heliyon. 2020;6(9): e04964. https://doi.org/10.1016/j.heliyon.2020.e04964.CrossRefPubMedPubMedCentral

Rock WP, Weatherill S, Anderson RJ. Retention of three fissure sealant resins. The effects of etching agent and curing method. Results over 3 years. Br Dent J. 1990;168(8):323–5. https://doi.org/10.1038/sj.bdj.4807185.CrossRefPubMed

Salas-López EK, Pierdant-Pérez M, Hernández-Sierra JF, et al. Effect of silver nanoparticle-added pit and fissure sealant in the prevention of dental caries in children. J Clin Pediatr Dent. 2017;41:48–52. https://doi.org/10.17796/1053-4628-41.1.48.CrossRefPubMed

Schill H, Gräser P, Bücher K, et al. 3-Year clinical performance of a new pit and fissure sealant. J Clin Med. 2022;11(13):3741. https://doi.org/10.3390/jcm11133741.CrossRefPubMedPubMedCentral

Simonsen RJ. Pit and fissure sealant: then, now, and next steps. J Public Health Dent. 2004;64:17–22.CrossRef

Smitha M, Paul ST, Nagaraj T, et al. Comparison and clinical evaluation of two pit and fissure sealants on permanent mandibular first molars: an in vivo study. J Contemp Dent Pract. 2019;20(10):1151–8.CrossRefPubMed

Subramaniam P, Konde S, Mandanna DK. Retention of a resin-based sealant and a glass ionomer used as a fissure sealant: a comparative clinical study. J Indian Soc Pedod Prev Dent. 2008;26(3):114–20. https://doi.org/10.4103/0970-4388.43192.CrossRefPubMed

Thitasomakul S, Tianviwat S. A cluster randomized controlled trial of the dental sealants quality in rural schoolchildren using innovative suction without dental assistance. Int J Environ Res Public Health. 2023;20:4035. https://doi.org/10.3390/ijerph20054035.CrossRefPubMedPubMedCentral

Tianviwat S, Chongsuvivatwong V, Sirisakulveroj B. Loss of sealant retention and subsequent caries development. Community Dent Health. 2008;25(4):216–20.PubMed

Wright JT, Crall JJ, Fontana M, et al. Evidence-based clinical practice guideline for the use of pit-and-fissure sealants: a report of the American Dental Association and the American Academy of Pediatric Dentistry. J Am Dent Assoc. 2016;147:672-682.e12. https://doi.org/10.1016/j.adaj.2016.06.001.CrossRefPubMed

Yildiz E, Dörter C, Efes B, Koray F. A comparative study of two fissure sealants: a 2-year clinical follow-up. J Oral Rehabil. 2004;31:979–84. https://doi.org/10.1111/j.1365-2842.2004.01334.x.CrossRefPubMed

Zhang XF, Liu ZG, Shen W, Gurunathan S. Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. Int J Mol Sci. 2016;17:1534. https://doi.org/10.3390/ijms17091534.CrossRefPubMedPubMedCentral

Title: Comparison of retention rates of silver nanoparticles-based fissure sealant and conventional resin-based sealant: a one-year randomized clinical trial
Authors: S. Svetha
K. Bansal
V. P. Mathur
N. Tewari
R. Morankar
M. Kalaivani
Publication date: 05-03-2025
Publisher: Springer Berlin Heidelberg
Keyword: Caries
Published in: European Archives of Paediatric Dentistry
Print ISSN: 1818-6300
Electronic ISSN: 1996-9805
DOI: https://doi.org/10.1007/s40368-025-01014-9

Podcast | Sexual health in older age

Springer Medicine

Comparison of retention rates of silver nanoparticles-based fissure sealant and conventional resin-based sealant: a one-year randomized clinical trial

Abstract

Background

Aim

Methods

Results

Conclusion

Trial registration number

Podcast | Sexual health in older age

Springer Medicine

Abstract

Background

Aim

Methods

Results

Conclusion

Trial registration number

Please log in to get access to this content