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Open Access 04-07-2024 | Original Contribution

Bone marrow cells contribute to seven different endothelial cell populations in the heart

Authors: Parisa Shabani, Vahagn Ohanyan, Ammar Alghadeer, Daniel Gavazzi, Feng Dong, Liya Yin, Christopher Kolz, Lindsay Shockling, Molly Enrick, Ping Zhang, Xin Shi, William Chilian

Published in: Basic Research in Cardiology | Issue 4/2024

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Abstract

Understanding the mechanisms underlying vascular regeneration in the heart is crucial for developing novel therapeutic strategies for myocardial ischemia. This study investigates the contribution of bone marrow-derived cells to endothelial cell populations in the heart, and their role in cardiac function and coronary circulation following repetitive ischemia (RI). Chimeric rats were created by transplanting BM cells from GFP female rats into irradiated male recipients. After engraftment chimeras were subjected to RI for 17 days. Vascular growth was assessed from recovery of cardiac function and increases in myocardial blood flow during LAD occlusion. After sorting GFP+ BM cells from heart and bone of Control and RI rats, single-cell RNA sequencing was implemented to determine the fate of BM cells. Our in vivo RI model demonstrated an improvement in cardiac function and myocardial blood flow after 17 days of RI with increased capillary density in the rats subjected to RI compared to Controls. Single-cell RNA sequencing of bone marrow cells isolated from rats' hearts identified distinct endothelial cell (EC) subpopulations. These ECs exhibited heterogeneous gene expression profiles and were enriched for markers of capillary, artery, lymphatic, venous, and immune ECs. Furthermore, BM-derived ECs in the RI group showed an angiogenic profile, characterized by upregulated genes associated with blood vessel development and angiogenesis. This study elucidates the heterogeneity of bone marrow-derived endothelial cells in the heart and their response to repetitive ischemia, laying the groundwork for targeting specific subpopulations for therapeutic angiogenesis in myocardial ischemia.
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Metadata
Title
Bone marrow cells contribute to seven different endothelial cell populations in the heart
Authors
Parisa Shabani
Vahagn Ohanyan
Ammar Alghadeer
Daniel Gavazzi
Feng Dong
Liya Yin
Christopher Kolz
Lindsay Shockling
Molly Enrick
Ping Zhang
Xin Shi
William Chilian
Publication date
04-07-2024
Publisher
Springer Berlin Heidelberg
Published in
Basic Research in Cardiology / Issue 4/2024
Print ISSN: 0300-8428
Electronic ISSN: 1435-1803
DOI
https://doi.org/10.1007/s00395-024-01065-x

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