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Open Access 01-12-2024 | Bladder Cancer | Research

Ferroptosis-related lncRNA AL136084.3 is associated with NUPR1 in bladder cancer

Authors: Jing Zhou, Li Zhang, Hao Wu, Sheng-Lin Gao, Xiao-Ping Chen, Li-Feng Zhang, Cui-Ping Zhao, Bing-Bing Wei, Yu Bai

Published in: Discover Oncology | Issue 1/2024

Abstract

Background

LncRNAs are critical regulators of bladder cancer (BLCA), and ferroptosis is a newly discovered cell death responsible for mediating apoptosis and tumorigenesis. The present study aims to establish a prognostic signature of differentially expressed ferroptosis-related lncRNAs (DEFRlncRNAs) and explore the DEFRlncRNA associated with NUPR1 in BLCA.

Methods

DEFRlncRNAs in BLCA patients were screened using univariate and multivariate Cox and LASSO regression analyses. In vitro experiments were performed to detect the regulatory effects of DEFRlncRNAs on BLCA cells. A prognostic signature of DEFRlncRNAs in BLCA was created and validated. Moreover, we used RNA-binding protein immunoprecipitation (RIP) to evaluate the correlated DEFRlncRNA with NUPR1.

Results

A prognostic signature involving 18 DEFRlncRNAs in BLCA was created. Overexpression of AL355353.2 or knockdown of AL136084.3 promoted apoptosis in 5637 and T24 cells in vitro. Results from Starbase database estimated that AL136084.3 was positively associated with NUPR1 (R = 0.229, p < 0.001). RIP analysis revealed the reciprocal binding of NUPR1 and AL136084.3 in BLCA.

Conclusion

The identified FRlncRNA pair signature has a good prognostic and clinical predictive value. The ferroptosis-related lncRNA AL136084.3 is correlated with NUPR1 in BLCA.

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Title: Ferroptosis-related lncRNA AL136084.3 is associated with NUPR1 in bladder cancer
Authors: Jing Zhou
Li Zhang
Hao Wu
Sheng-Lin Gao
Xiao-Ping Chen
Li-Feng Zhang
Cui-Ping Zhao
Bing-Bing Wei
Yu Bai
Publication date: 01-12-2024
Publisher: Springer US
Keywords: Bladder Cancer
Bladder Cancer
Published in: Discover Oncology / Issue 1/2024
Print ISSN: 1868-8497
Electronic ISSN: 2730-6011
DOI: https://doi.org/10.1007/s12672-024-01564-2

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