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Open Access 25-04-2024 | Original Scientific Article

Biocompatibility and acid resistance of preformed crowns in children: an in vitro study

Authors: T. Hogerheyde, D. Coates, L. Walsh, S. Zafar

Published in: European Archives of Paediatric Dentistry

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Abstract

Purpose

To investigate the in vitro biocompatibility of human gingival fibroblasts with preformed paediatric crowns and resistance to acid exposure at levels that simulate the oral environment.

Methods

This laboratory study investigated primary HGFs viability, metabolic activity, cytotoxicity, and apoptotic events on preformed metal crown discs, composite resin-coated wells, and monolithic zirconia fragments at 24, 48, and 72 h using the ApoTox-Glo Triplex assay. The PPCs were also immersed in 0.1% lactic acid, 0.2% phosphoric acid, or 10% citric acid for 7 days at 37 °C to reproduce conditions associated with dietary intake or gastric reflux. Samples were then subject to inductively coupled plasma optical emission spectrometry to quantitate the release of ions.

Results

The viability of HGFs on stainless steel and CR significantly declined at 48 and 72 h, representing potential cytotoxicity (p < 0.05). Cytotoxicity of HGFs was also higher for stainless steel and ZR compared to control (p < 0.05). PMCs and ZR crowns gave minimal ion release. Meanwhile, significant quantities of metallic ions, including copper (Cu), iron (Fe), nickel (Ni), and zinc (Zn), were present in eluates from veneered-preformed metal crowns.

Conclusion

As PPCs can be exposed to highly acidic environments for many years, thus the release of metallic ions from V-PMCs should form the further investigation in future studies.
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Metadata
Title
Biocompatibility and acid resistance of preformed crowns in children: an in vitro study
Authors
T. Hogerheyde
D. Coates
L. Walsh
S. Zafar
Publication date
25-04-2024
Publisher
Springer Berlin Heidelberg
Published in
European Archives of Paediatric Dentistry
Print ISSN: 1818-6300
Electronic ISSN: 1996-9805
DOI
https://doi.org/10.1007/s40368-024-00898-3