Skip to main content
Key Specialties
Anesthesiology Cardiology Dermatology Diabetology Endocrinology Gastroenterology General Medicine Geriatrics Hematology Infectious Diseases Internal Medicine Nephrology Neurology Obstetrics and Gynecology Oncology Ophthalmology Pediatrics Psychiatry Radiology Respiratory Medicine Rheumatology Surgery Urology
Congress Coverage
ASH 2024 Annual Meeting 2024 ESMO Congress 2024 ERS Congress 2024 EASD Congress 2024 ESC Congress 2024 EAN Congress 2024 ASCO Annual Meeting 2024 ACC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on adolescent vaping

LIVE: Wednesday 29th January, 18:00-19:30 CET

Growing numbers of young people are using e-cigarettes, despite warnings of respiratory effects and addiction. How can doctors tackle the epidemic, and what health effects should you prepare to manage in your clinics?
ADVANCED SEARCH
Log in
Top
Antimicrobial Resistance & Infection Control
Published in:

Open Access 01-12-2017 | Research

Antimicrobial susceptibility of Brucella melitensis in Kazakhstan

Authors: Alexandr Shevtsov, Marat Syzdykov, Andrey Kuznetsov, Alexandr Shustov, Elena Shevtsova, Kalysh Berdimuratova, Kasim Mukanov, Yerlan Ramankulov

Published in: Antimicrobial Resistance & Infection Control | Issue 1/2017

Login to get access

Abstract

Background

Kazakhstan belongs to countries with a high level of brucellosis among humans and farm animals. Although antibiotic therapy is the main way to treat acute brucellosis in humans there is still little information on a circulation of the antibiotic-resistant Brucella strains in the Central Eurasia. In this article we describe an occurrence of the drug resistance of Brucella melitensis isolates in Kazakhstan which is among the largest countries of the region.

Methods

Susceptibilities to tetracyclin, gentamycin, doxycyclin, streptomycin and rifampicin were investigated in 329 clinical isolates of Brucella melitensis using E-test method.

Results

All isolates were susceptible to streptomycin, tetracycline and doxycycline. 97.3% of the Brucella isolates were susceptible to gentamycin, although only 37.4% of isolates were susceptible to rifampicin. 21.9% of isolates had intermediate resistance, and 26.4% of isolates were resistant to this antibacterial drug.

Conclusion

Isolates of Brucella melitensis circulating in Kazakhstan are susceptible to streptomycin, doxicyclin, tetracyclin and gentamycin. At the same time the resistance to rifampicin is widespread, almost half of the isolates were rifampicin-resistant (including the intermediate resistance).
Literature
1.
Pappas G, Papadimitriou P, Akritidis N, Christou L, Tsianos EV. The new global map of human brucellosis. Lancet Infect Dis. 2006;6(2):91–9.CrossRefPubMed
2.
Mantur BG, Amarnath SK, Shinde RS. Review of clinical and laboratory features of human brucellosis. Indian J Med Microbiol. 2007;25(3):188–202.CrossRefPubMed
3.
Durusoy R, Karababa AO. Completeness of hepatitis, brucellosis, syphilis, measles and HIV/AIDS surveillance in Izmir, Turkey. BMC Public Health. 2010;10:71.CrossRefPubMedPubMedCentral
4.
Ravanel N, Gestin B, Maurin M. In vitro selection of fluoroquinolone resistance in Brucella melitensis. Int J Antimicrob Agents. 2009;34(1):76–81.CrossRefPubMed
5.
Nicoletti P. Brucellosis: past, present and future. Prilozi. 2010;31(1):21–32.PubMed
6.
Scholz HC, Revilla-Fernandez S, Al Dahouk S, Hammerl JA, Zygmunt MS, Cloeckaert A, Koylass M, Whatmore AM, Blom J, Vergnaud G, et al. Brucella vulpis sp. nov., isolated from mandibular lymph nodes of red foxes (Vulpes Vulpes). Int J Syst Evol Microbiol. 2016;66(5):2090–8.PubMed
7.
Eschenbrenner M, Wagner MA, Horn TA, Kraycer JA, Mujer CV, Hagius S, Elzer P, DelVecchio VG. Comparative proteome analysis of Brucella melitensis vaccine strain rev 1 and a virulent strain, 16M. J Bacteriol. 2002;184(18):4962–70.CrossRefPubMedPubMedCentral
8.
Diaz Aparicio E. Epidemiology of brucellosis in domestic animals caused by Brucella melitensis, Brucella suis and Brucella abortus. Rev Sci Tech. 2013;32(1):43–51. 53-60CrossRefPubMed
9.
Galinska EM, Zagorski J. Brucellosis in humans - etiology, diagnostics, clinical forms. Ann Agr Env Med. 2013;20(2):233–8.
10.
Yousefi-Nooraie R, Mortaz-Hejri S, Mehrani M, Sadeghipour P. Antibiotics for treating human brucellosis. Cochrane Database Syst Rev. 2012;10:CD007179.PubMed
11.
Shevtsova E, Shevtsov A, Mukanov K, Filipenko M, Kamalova D, Sytnik I, Syzdykov M, Kuznetsov A, Akhmetova A, Zharova M, et al. Epidemiology of brucellosis and genetic diversity of Brucella abortus in Kazakhstan. PLoS One. 2016;11(12):e0167496.CrossRefPubMedPubMedCentral
12.
Saltoglu N, Tasova Y, Inal AS, Seki T, Aksu HS. Efficacy of rifampicin plus doxycycline versus rifampicin plus quinolone in the treatment of brucellosis. Saudi Med J. 2002;23(8):921–4.PubMed
13.
Geyik MF, Gur A, Nas K, Cevik R, Sarac J, Dikici B, Ayaz C. Musculoskeletal involvement of brucellosis in different age groups: a study of 195 cases. Swiss Med Wkly. 2002;132(7-8):98–105.PubMed
14.
Corbel MJ. Brucellosis in humans and animals. Geneva: World Health Organization; 2006.
15.
Ariza J, Gudiol F, Pallares R, Viladrich PF, Rufi G, Corredoira J, Miravitlles MR. Treatment of human brucellosis with Doxycycline plus Rifampin or Doxycycline plus streptomycin - a randomized, double-blind-study. Ann Intern Med. 1992;117(1):25–30.CrossRefPubMed
16.
Bayram Y, Korkoca H, Aypak C, Parlak M, Cikman A, Kilic S, Berktas M. Antimicrobial susceptibilities of Brucella isolates from various clinical specimens. Int J Med Sci. 2011;8(3):198–202.CrossRefPubMedPubMedCentral
17.
Kinsara A, Al-Mowallad A, Osoba AO. Increasing resistance of Brucellae to co-trimoxazole. Antimicrob Agents Chemother. 1999;43(6):1531.PubMedPubMedCentral
18.
De Rautlin de la Roy YM, Grignon B, Grollier G, Coindreau MF, Becq-Giraudon B. Rifampicin resistance in a strain of Brucella melitensis after treatment with doxycycline and rifampicin. J Antimicrob Chemother. 1986;18(5):648–9.CrossRefPubMed
19.
Castaneda MR. A practical method for routine blood cultures in brucellosis. Proc Soc Exp Biol Med. 1947;64(1):114.CrossRefPubMed
20.
21.
Lopez-Goni I, Garcia-Yoldi D, Marin CM, de Miguel MJ, Munoz PM, Blasco JM, Jacques I, Grayon M, Cloeckaert A, Ferreira AC, et al. Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains. J Clin Microbiol. 2008;46(10):3484–7.CrossRefPubMedPubMedCentral
22.
Jorgensen JH, Hindler JA, Bernard K. Methods of antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria.Approved guidelines - second edition, vol. 30. Wayne, PA: Clinical and Laboratory Standard Institute; 2010.
23.
CLSI. Performance standard for antimicrobial susceptibility testing: twenty-fourth informational supplement.CLSI document M100-S24, vol. 34. Wayne, PA: Clinical and Laboratory Standard Institute; 2014.
24.
Organization WH. Global tuberculosis report 2013. Geneva: World Health Organization; 2013.
25.
Terlikbayeva A, Hermosilla S, Galea S, Schluger N, Yegeubayeva S, Abildayev T, Muminov T, Akiyanova F, Bartkowiak L, Zhumadilov Z, et al. Tuberculosis in Kazakhstan: analysis of risk determinants in national surveillance data. BMC Infect Dis. 2012;12:262.CrossRefPubMedPubMedCentral
26.
Ariza J, Bosilkovski M, Cascio A, Colmenero JD, Corbel MJ, Falagas ME, Memish ZA, Roushan MR, Rubinstein E, Sipsas NV, et al. Perspectives for the treatment of brucellosis in the 21st century: the Ioannina recommendations. PLoS Med. 2007;4(12):e317.CrossRefPubMedPubMedCentral
27.
Barbosa Pauletti R, Reinato Stynen AP, Pinto da Silva Mol J, Seles Dorneles EM, Alves TM, de Sousa Moura Souto M, Minharro S, Heinemann MB, Lage AP. Reduced susceptibility to Rifampicin and resistance to multiple antimicrobial agents among Brucella abortus isolates from cattle in Brazil. PLoS One. 2015;10(7):e0132532.CrossRefPubMedPubMedCentral
28.
Denk A, Demirdag K, Kalkan A, Ozden M, Cetinkaya B, Kilic SS. In vitro activity of Brucella melitensis isolates to various antimicrobials in Turkey. Infect Dis (Lond). 2015;47(6):364–9.CrossRef
29.
Abdel-Maksoud M, House B, Wasfy M, Abdel-Rahman B, Pimentel G, Roushdy G, Dueger E. In vitro antibiotic susceptibility testing of Brucella isolates from Egypt between 1999 and 2007 and evidence of probable rifampin resistance. Ann Clin Microbiol Antimicrob. 2012;11:24.CrossRefPubMedPubMedCentral
Metadata
Title
Antimicrobial susceptibility of Brucella melitensis in Kazakhstan
Authors
Alexandr Shevtsov
Marat Syzdykov
Andrey Kuznetsov
Alexandr Shustov
Elena Shevtsova
Kalysh Berdimuratova
Kasim Mukanov
Yerlan Ramankulov
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Antimicrobial Resistance & Infection Control / Issue 1/2017
Electronic ISSN: 2047-2994
DOI
https://doi.org/10.1186/s13756-017-0293-x

Other articles of this Issue 1/2017

Prevalence of antimicrobial resistant pathogens from blood cultures: results from a laboratory based nationwide surveillance in Ghana

  • Open Access
  • Research

MRSA decolonization failure—are biofilms the missing link?

  • Open Access
  • Research

Co-existence of bla OXA-23 and bla NDM-1 genes of Acinetobacter baumannii isolated from Nepal: antimicrobial resistance and clinical significance

  • Open Access
  • Research

High rates of multidrug resistance among uropathogenic Escherichia coli in children and analyses of ESBL producers from Nepal

  • Open Access
  • Research

High prevalence of the mcr-1 gene in retail chicken meat in the Netherlands in 2015

  • Open Access
  • Letter to the Editor

Sites of colonization in hospitalized patients with infections caused by extended-spectrum beta-lactamase organisms: a prospective cohort study

  • Open Access
  • Research

Keynote webinar | Spotlight on menopause

Menopause can have a significant impact on the body, with effects ranging beyond the endocrine and reproductive systems. Learn about the broader systemic effects of menopause, so you can help patients in your clinics through the transition.   

Prof. Martha Hickey
Dr. Claudia Barth
Dr. Samar El Khoudary
Developed by: Springer Medicine
Watch now

Keynote webinar | Spotlight on adolescent vaping

  • Live
  • Webinar | 29-01-2025 | 18:00 (CET)

Growing numbers of young people are using e-cigarettes, despite warnings of respiratory effects and addiction. How can doctors tackle the epidemic, and what health effects should you prepare to manage in your clinics?

Watch it live: Wednesday 29th January, 18:00-19:30 CET
 

Prof. Ann McNeill
Dr. Debbie Robson
Benji Horwell
Developed by: Springer Medicine
Join the webinar

Keynote webinar | Spotlight on modern management of frailty

Frailty has a significant impact on health and wellbeing, especially in older adults. Our experts explain the factors that contribute to the development of frailty and how you can manage the condition and reduce the risk of disability, dependency, and mortality in your patients.

Prof. Alfonso Cruz-Jentoft
Prof. Barbara C. van Munster
Prof. Mirko Petrovic
Developed by: Springer Medicine
Watch now

A quick guide to ECGs

Improve your ECG interpretation skills with this comprehensive, rapid, interactive course. Expert advice provides detailed feedback as you work through 50 ECGs covering the most common cardiac presentations to ensure your practice stays up to date. 

PD Dr. Carsten W. Israel
Developed by: Springer Medizin
Start the cases

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine
Read more
Image Credits