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26-12-2023 | Acute Lymphoblastic Leukemia | Original Article

SPAG6 regulates cell proliferation and apoptosis via TGF-β/Smad signal pathway in adult B-cell acute lymphoblastic leukemia

Authors: Beibei Zhao, Jiaxiu Yin, Li Ding, Jie Luo, Jing Luo, Jiao Mu, Shirui Pan, Juan Du, Yirui Zhong, Linyi Zhang, Lin Liu

Published in: International Journal of Hematology | Issue 2/2024

Abstract

Adult B-cell acute lymphoblastic leukemia (B-ALL) prognosis remains unsatisfactory, and searching for new therapeutic targets is crucial for improving patient prognosis. Sperm-associated antigen 6 (SPAG6), a member of the cancer-testis antigen family, plays an important role in tumors, especially hematologic tumors; however, it is unknown whether SPAG6 plays a role in adult B-ALL. In this study, we demonstrated for the first time that SPAG6 expression was up-regulated in the bone marrow of adult B-ALL patients compared to healthy donors, and expression was significantly reduced in patients who achieved complete remission (CR) after treatment. In addition, patients with high SPAG6 expression were older (≥ 35 years; P = 0.015), had elevated white blood cell counts (WBC > 30 × 10⁹/L; P = 0.021), and a low rate of CR (P = 0.036). We explored the SPAG6 effect on cell function by lentiviral transfection of adult B-ALL cell lines BALL-1 and NALM-6, and discovered that knocking down SPAG6 significantly inhibited cell proliferation and promoted apoptosis. We identified that SPAG6 knockdown might regulate cell proliferation and apoptosis via the transforming growth factor-β (TGF-β)/Smad signaling pathway.

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Title: SPAG6 regulates cell proliferation and apoptosis via TGF-β/Smad signal pathway in adult B-cell acute lymphoblastic leukemia
Authors: Beibei Zhao
Jiaxiu Yin
Li Ding
Jie Luo
Jing Luo
Jiao Mu
Shirui Pan
Juan Du
Yirui Zhong
Linyi Zhang
Lin Liu
Publication date: 26-12-2023
Publisher: Springer Nature Singapore
Keyword: Acute Lymphoblastic Leukemia
Published in: International Journal of Hematology / Issue 2/2024
Print ISSN: 0925-5710
Electronic ISSN: 1865-3774
DOI: https://doi.org/10.1007/s12185-023-03684-x

EASD 2024

Springer Medicine

SPAG6 regulates cell proliferation and apoptosis via TGF-β/Smad signal pathway in adult B-cell acute lymphoblastic leukemia

Abstract

EASD 2024

Springer Medicine

Abstract

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